Tuesday, May 20, 2014

How Genetic Modification is done by Gloria Poole, RN, artist of Missouri

Genetic modification and manipulation of cells 
written by Gloria Poole, RN, artist of Missouri and Georgia. 

I am quoting a medical textbook and will include info about it but here's the quotes: " DNA has become the easier [ to analyze]. It is now possible  o excise [cut out] specific regions of DNA to obtain them in virtually unlimited quantities, and to determine the sequence of their nucleotides at a rate of hundreds of nucleotides  a day. By variations of the same techniques, an isolated gene can be altered [engineered] at will and transferred back into cells in culture  or [with rather more difficulty ] into the germ line of animals where the modified gene becomes incorporated [ becomes a part of] as a permanent functional part of the genome. " page 181. And continuing further down the same page, this quote: " ..recombinant DNA technology has generated new experimental approaches that have revolutionized cell biology. " And skip to page 194 same textbook, for this quote: " although it is often not difficult to obtain mutants that are deficient in a particular process, such a DNA replication or eye development, it can take many years to trace the deficit to a particular altered protein. Recently, recombinant DNA technology has made possible  a different genetic approach in which one starts with a protein and creates a mutant cell or organism in which that protein [or its expression} is abnormal. Because the new approach reverses  the traditional gene-to-protein direction of genetic analysis, it is commonly referred to as reverse genetics.  Reverse genetics begins with a protein with interesting properties that has been isolated from a cell. By methods described [in a following chapter] the gene encoding the protein is cloned and its nucleotide sequence is determined. This sequence is then altered by biochemical  means, to create a mutant gene that codes for an altered version of the protein. The mutant gene is transferred into a cell, where it can integrate into a chromosome  by genetic recombination to become part of the cells' genome. If the gene is expressed, the cell and all of its descendants will now synthesize the an altered protein. if the original cell used for gene transfer is a fertilized egg, whole multi-cellular organisms can be obtained that contain the mutant gene, and some of these transgenic organisms will pass the [mutant] gene on to their progeny [offspring] as a permanent part of their germ line. Such genetic transformations are now routinely performed on such organisms as complex as fruit flies and mammals. And note that biotech is also doing these experiments on humans with cells obtained from aborted babies and their ovaries and gonads.]  Technically, even humans could now be transformed in this way..." 

I read some of the chapter explaining how the manipulation of the cells is done. It is very complex and difficult reading because it is geared to the education of medical doctors [but I, Gloria Poole, RN own this textbook that I got for medical education for my purposes.] I am only going to summarize a few key points in the how they do it section because I am writing this so lay people without any medical training or skills could understand it.  From pages 207-209 same textbook: The "scientists"  hydrolyze [tear apart} the amino acids [proteins] and then they manipulate  them using enzymes called aminoacyl-tRNA syntheses but there is a different enzyme for all 20 amino acids. They add "two methyl groups to G: n, N-dimethyl G; two hydrogens  to U: dihydro U; isopentenyl to A: N to the 6th power and [chemical sign of a triangle [not able to make on this keyboard] to the 2nd power isopentenyl] A; and sulphur to replace the oxygen in U : 4-thiouridine.  Then the amino acids recombine with a different tRNA molecule and is modified. The scientists can cause the cells to degenerate  by transforming it to more than one tRNA  or that a single tRNA can pair with more than one codon [ a triplet of three nucleotides in the mRNA molecule. I do not remember chemistry well enough to understand it all myself. The medical textbook I quoted is : Molecular Biology of The Cell' second edition"  written by Bruce Alperts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts and James D Watson who are all PhD's except for one who is Martin Raff educated at McGill University who is a M.D.  it was published by Garland Publishing, Inc of New York and London.

I am writing this because I am OPPOSED to the manipulation of human cells for any reason. And I am OPPOSED to the manipulation / genetic modification of foods, seeds, animals also. 

I am not copyrighting this particular post because it contains quoted material from a medical textbook. But most of what I personally write is copyrighted content. See the about me page here: http://gloriapoole-rn-artist.blogspot.com/p/about-gloria-oole-rn-artist-of-missouri.html 
for more information. Also see my copyright notice on my official blog of Tapestry of LIFE at https://tapestry-of-life-llp.blogspot.com and on my name blog https://gloriapoole.blogspot.com.

Gloria Poole, RN, artist / Gloria Poole / Gloria / gloriapoole / gloria-poole / gloria.poole / artist-gloriapoole / gloriapoole_RN / gloriapoole.RN / at my own apt in Missouri 20 May 2014 at 10:27am. 

Friday, May 9, 2014

Study of words by Gloria Poole-RN- artist of Missouri and Georgia

This post is about the importance of words. There are many people who think that they can say anything and that it doesn't matter how much they deceive, lie, trick, scam others. But it matters. I looked up the truth from the Holy Bible and these are only some of the scriptures about words. And yes, of course, this pertains to the prolife cause and to the battle by Christians against the attempts by evil groups to turn the U S away from GOD.  In the prolife battle, the main instrument the wicked killers of the preborn use, is lies, lies and more lies to fool foolish women who don't know the scriptures.

 If you read each one of these, you will be transformed about your duty to control your words to not speak evil.

It is written, " Neither have I gone back from the commandment of His lips; I have esteemed the words of His [GOD's] mouth more than my necessary food." Job 23:12

"All the while my breath is in me, and The Spirit of GOD is in my nostrils, my lips shall not speak wickedness, nor my tongue utter deceit." Job 27:18

"Blessed is the man who walketh not in the counsel [advice, legal advice] of the ungodly, nor standeth in the seat of sinners, nor sitteth in the seat of the scornful.  But his delight is in the law of THE LORD; and in His law, doth he meditate day and night." Psalms 1:1-2

"The words of THE LORD are pure words, as silver tried in a furnace purified seven times. " Psalm 12: 6

"How sweet are thy words unto my taste! yea, sweeter than honey to my mouth. "Psalms 119:103

"Thy word is a lamp unto my feet and a light unto my path. " Psalms 119:105

"A wise man will hear and will increase learning; and a man of understanding shall attain unto wise counsels; to understand a proverb and the interpretation; the words of the wise and their dark sayings. The fear of THE LORD is the beginning of knowledge; but fools despise wisdom and instruction. " Proverbs 1: 5-7

"The words of the wicked are to lie in wait for blood; but the mouth of the upright shall deliver them. The wicked are overthrown and are not; but the house of the righteous shall stand. " Proverbs 12: 6-7

"The thoughts of the wicked are an abomination to THE LORD; but the words of the pure are pleasant words.". Proverbs 15: 26

"Pleasant words are as an honeycomb, sweet to the soul, and health to the bones. " Proverbs 16: 24

"That I might make thee know the certainty of the words of truth, that thou mightiest answer the words of truth to them that send unto thee." Proverbs 22:23

"The words of the wise are as goads, and as nails fastened by the masters of assemblies  which are given from one shepherd.  And further , by these..be admonished; of making many books there is no end; and of much study is a weariness of the flesh.  Let us hear the conclusion of the whole matter: fear GOD, and keep HIS commandments [words] ; for this is the whole duty of man. For GOD shall bring every work into judgement, with every secret thing, whether it be good or whether it be evil. "Eccelesiates 12:11-14

"The instruments also of the churl [defined as " a low-bred countryman; a surly person, a miser] are evil; he deviseth wicked devices to destroy the poor with lying words, even when the needy speaketh right. " Isaiah 32: 7

"Then THE LORD put forth HIS hand and touched my mouth, and THE LORD said unto me, "behold, I have put My words in thy mouth." Jeremiah 1:9

"Then THE LORD said unto me, " proclaim all these words in the cities of Judah, and in the streets of Jerusalem, saying, 'hear ye the words of this covenant, and do them'. " Jeremiah 11: 6

"The word which came to Jeremiah from THE LORD, saying, 'arise, and go down to the potter's house and there I will cause thee to hear My words. " Jeremiah 18:1-2

"And thou shalt say unto them, 'Thus saith THE LORD: if ye will not hearken to Me, to walk in My law, which I have set before you, to hearken to the words of My servants the  prophets whom I sent unto you, both rising up early and sending them, but ye have not hearkened, then will I make this house like Shi-loh, and will make this city a curse to all the nations of the earth. " Jeremiah 26: 4-6

JESUS said, "For by thy words thou shalt be justified and by thy words thou shalt be condemned. ". Matthew 12:37

JESUS said, "heaven and earth shall pass away but My words shall not pass away." Mark 13:31

All scripture from The King James Holy Bible.

Posted by me Gloria Poole, RN, artist of Missouri and Georgia; from my own private apt in Missouri, 9-May-2014 at 12:09pm.